constrain.lab.transformation

This part of the lab module is used for making transformations

Functions

ODtime(initialOD, time[, td])	Calculates the OD based on doupling time.
calculate_volume_and_total_concentration(...)	Calculates the volume and total concentration of a list of DNA parts.Parameters :2: (WARNING/2) Title underline too short. Calculates the volume and total concentration of a list of DNA parts.Parameters ---------- amplicons list A list of amplicon objects amplicon_parts_amounts_total dict A dictionary of amplicon names and their respective total amounts n int (optional) Gives the option of multiplying the volume is needed. Optional set to 1.
ng_to_nmol(ng, bp)	Calculates nanogram to nanomol for transformation mixes.
pool_parts(amplicons, part_names, ...)	Pools amplicon parts and returns a dictionary of pooled volumes.
print_pooled_parts(pooled_volumes)	Prints the pooled parts and calculated concentrations.
time_to_inculate([initialOD, td, verbose, ...])	Calculates when a starter culture is ready to be inoculated
transformation_mix(reaction_names, ...[, media])	This function makes a pandas dataframe of the parts(their location)
transformation_partitipants(...[, amnt, ...])	Returns a dict with the µl amounts needed in a transformation reaction.
wanted_mass(wanted_moles, size)	param wanted_moles: wanted moles in nmol
wanted_volume(wanted_mass, actual_concentration)	param wanted_mass: wanted mass in ng