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Welcome to ConStrain’s documentation!

Our mission is to revolutionize the way in which we make biology. How do we do that? We pursue reproducible high-throughput strain construction by adopting literate programming with ConStrain. We hope you want to be part of the revolution. Below you will find documentation for how to install and use ConStrain.

Suppose you are interested in examples, head over to our notebooks. Feel free to reach out if something is unclear.

Features of ConStrain

  • Combinatorial library generation

  • HT cloning and transformation workflows

  • Flowbot One instructions

  • CSV-based LIMS system as well as integration to Benchling

  • Genotyping of microbial strains

  • Advanced Machine Learning of biological datasets with the AutoML H2O

  • Workflows for selecting enzyme homologs

  • Promoter selection workflows from RNA-seq datasets

  • Data analysis of large LC-MS datasets along with workflows for analysis

Getting started

To get started with making microbial strains in an HT manner please follow the steps below:

  1. Install ConStrain. You will find the necessary information below under installation.

  2. Check out our notebooks for inspiration to make HT strain construction with ConStrain.

  3. You can start making your own workflows by importing ConStrain into either Google colab or Jupyter lab/notebooks.

Indices and tables